Content Determination of Naringin in Jizhi Droppills by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish HPLC method for the determination of naringin in Jizhi droppills. Methods Kromasil C18 (4.6 mm?200 mm, 5 ?m) was used. The mobile phase was consisted of acetonitrile- 1% acetate solution (40∶60). The flow rate was 1.0 mL/min. The UV detection wavelength was at 283 nm. Results The linear range for naringin was 0.07～0.35 ?g, r=0.999 8. The RSD of precision test was 0.3%. The RSD of stability test was 2.3%. The RSD of repeatability test was 1.29%. The average recovery was 98.9%. Conclusion The method of precision and recovery was high, and stability and repeatability was good.