Exogenous nitric oxide inhibits proliferation of in vitro cultured human keloid fibroblasts / 重庆医科大学学报

ABSTRACT

Objective:To explore the inhibitive effects of exogenous nitric oxide on the proliferation of in vitro cultured human keloid fibroblasts.Methods:Fibroblasts was isolated from fresh pathological scar tissue and cultured.Fibroblasts were distributed in five groups.Fibroblasts in control group A was cultured without SNP;in experiment group B～D,SNP was used in the concentration of 100、200、300 ?mol/L each;in experiment group E,Fibroblasts was cultured with 200 ?mol/L SNP and 10 ?mol/L methylene blue.After 24h,Griess and MTT were used to detect the concentration of NO and cell vigor.Results:The release of NO increased along with the enhancement of the concentration of SNP.The amount of living cells in experiment groups decreased compared with the controls as shown by MTT tes(tP