Upregulation of VEGF and downregulation of IL-1?、TNF-? by edaravone in rats with vascular cognitive impairment / 重庆医科大学学报

ABSTRACT

Objective:To investigate the influence of edaravone on expression of VEGF,IL-1? and TNF-? in vascular cognitive impairment brain and the serum level of VEGF,IL-1? and TNF-? in vascular cognitive impairment rats,in order to study neuroprotection mechanism of edaravone against vascular cognitive impairment.Methods:The vacsular cognitive impairment rat model was established by permanent bilateral occlusion of both common carotid arteries.Behaviour was evaluated 2 weeks after permanent bilateral occlusion of both common carotid arteries with Morris water maze and shuttle box task.Wistar rats were randomly divided into sham-operation group(S),vascular cognitive impairment model group(M)and edaravone treatment group(MT).Serum level and expression of IL-1? and TNF-? was measured by enzyme linked immunosorbent assay,VEGF by enzyme linked immunosorbent assay and western blotting.Results:Edaravone significantly improved cognitive impairment from permanent bilateral occlusion of both common carotid arteries.The serum level and expression of IL-1? and TNF-? in MT group were lower than these in M group(P