Effect of Tongbiling on VEGF mRNA Expression in Rheumatoid Arthritis Synovial Cell / 中国中医药信息杂志

ABSTRACT

Objective To study effect of Tongbiling (TBL) on VEGF mRNA expression in RA synovlcytes cultured in vitro and explore its mechanism. Method Synovial cells from the knee joint in patient with RA were digested, divided and separately cultured after arthroscopy. Blank controlsDMEM culture containing 10% FBS in saline;Low-dose admission group of TBLDMEM culture containing 10% FBS in 50 mg/L TBL;High-dose admission of TBLDMEM culture containing 10% FBS in 200 mg/L TBL. RNA were distilled. Expression of VEGF mRNA were detected with RT-PCR. Result Compared with control group, expression of VEGF mRNA in low and high-dose admission groups of TBL both decreased (P