Study on MTHFR C677T Polymorphism in Ischemic Stroke with Blood Stasis Syndrome / 中国中医药信息杂志

ABSTRACT

Objective To explore the relationship between the C677T single nucleotide polymorphism of N5, N10-methylenetetrahydrofolate reductase (MTHFR) and blood stasis syndrome (BSS) of ischemic stroke (IS). Methods The MTHFR C677T genotypes in 84 patients of IS of BSS type (BSS-IS group), 143 patients of IS of non-BSS type (non-BSS-IS group) were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Results The constituent ratio of MTHFR CC, CT and TT genotype was significantly different between two groups (? 2 =12.618;d.f.=2, P=0.002). The frequencies of T allele in patients with BSS-IS (39.29%) were higher than that of non-BSS-IS group (24.48%). The MTHFR TT genotype was associated with a 3.730-fold increased risk for BSS-IS (OR=3.730;95%CI, 1.229～11.318; P =0.014). Conclusion The MTHFR TT genotype may be one of the risk factors associated with the susceptivity to BSS-IS.