Structural features of polysaccharide MP-3b from Mulberry leaves / 中成药

ABSTRACT

AIM: To characterize the chemical structure of a homogenous polysaccharide MP-3b obtained from Mulberry leaves. METHODS: Sugar component analysis,methylation analysis,partial acid hydrolysis,IR,ESI-MS,NMR were applied to determine the structural features. RESULTS: The molecular weight of MP-3b was 8.9?10~4 dalton by HPGFC.MP-3b consisted of rhammose,arabinose,xylose,glucose,galactose and galacturonic acid units in the molar ratio of 0.651.000.230.420.980.60.The chain of MP-3b included terminal,1,5-linked,1,3,5-linked Ara and terminal,1,2-linked,1,2,4-linked Rha,and 1,4-linked Xyl,and terminal Glc,and terminal,1,3-linked,1,6-linked,1,3,6-linked Gal and 1,4-linked,1,3,4-linked GalA.The side chain contained branched(1→5) linked arabinan. CONCLUSION: MP-3b is a new acidic polysaccharide obtained from Mulberry leaves for the first time.