Determination of Astragaloside Ⅳ in Fufang Buqi Yangxue Oral Solution by RP-HPLC / 中药新药与临床药理
Article
in Zh
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| ID: wpr-579365
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WPRO
ABSTRACT
Objective To establish the method for determination of astragaloside Ⅳ in Fufang Buqi Yangxue Oral Solution by RP-HPLC.Methods The content of astragaloside Ⅳ was determined by a HPLC system under the following conditions:the chromatographic column was Agilent C18(150 mm? 4.60 mm,5? m),the mobile phase was acetonitrile-water(60:40),the flow rate was 1.0 mL? min-1,and the detection wavelength was at 203 nm.Results Astragaloside Ⅳ showed good linearity in the range of 0.34~ 3.40 ?g(r=0.999 9).The average recovery was 100.8 %,and RSD was 1.47 %(n=6).Conclusion The HPLC method is simple and reproducible,and can be used for quality control of Fufang Buqi Yangxue Oral Solution.
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Index:
WPRIM
Language:
Zh
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Type:
Article