Analysis of coumarins in extract of Cnidium monnieri by ultra-performance liquid chromatographic coupled to electrospray ionization time of flight mass/mass spectrometry / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-579476
ABSTRACT
AIM:
To set up a rapid analysis and characterization method for coumarins in extract of Cnidium monnieri.METHODS:
The sample was analyzed by ultra-performance liquid chromatography with a reversed phase C_(18) column using a binary eluent of acetonitrile and water(with 0.1% formic acid) under gradient conditions.The ~+ ions of coumarins in Cnidium monnieri extract were observed by positive ion electrospray time of flight mass spectrometry (ESI-TOF-MS) online detection,and the fragmentation behavior obtained by ESI-TOF-MS/MS,then coumarins was identified through accurate molecular weight and molecular formula and fragmentation information combined with literature review.RESULTS:
All components could be separated rapidly within 10 min,and five coumarins-xanthotoxol,bergapten,xanthotoxin,imperatorin and osthol could be primary identified in Cnidium monnieri extract.CONCLUSION:
This method is effective for identification of coumarins in extract of Cnidium monnieri.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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