Identification of compounds in supercritical CO_2 extraction from Hypericum Perforatum L. by HPLC / 中成药

ABSTRACT

AIM: To identify and determine compounds in supercritical CO_2 extraction from Hypericum perforatum L. METHODS: The contents of flavones and hyperforin were determined by reverse phase HPLC.The condition in supercritical fluid extraction(SFE) was extraction temperature at 40 ?C,extraction pressure at 20 MPa,separation temperature at 45 ?C,separation presure at 8 MPa,extraction time 1 h. RESULTS: There was no flavones and the content of hyperforin was 180.6 mg/g in the sample of SFE using neat CO_2.The flavones and hyperforin were found in SFE extract using ethanol as modifier and the contents of rutin,quercetin,hyperoside,hyperforin were 1.10 mg/g、1.84 mg/g、1.33 mg/g、26.19 mg/g,respectively. CONCLUSION: The concentration of hyperforin by SFE with neat CO_2 is significantly higher than that with modified supercritical CO_2 extraction. The method of SFE with neat CO_2 can be used to prepare extracts with high concentration hyperforin.