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Construction of the standard curve for Real-time PCR detecting the expression of Angptl3 in rats / 重庆医科大学学报
Journal of Chongqing Medical University ; (12)2003.
Article in Chinese | WPRIM | ID: wpr-579662
ABSTRACT

Objective:

To construct the standard curve for real-time PCR detecting the tissue expression level of Angptl3 in SD rat.

Methods:

The total RNA of the SD rat's liver was the template.We got lots of the Angptl3 partial DNA sequence by RT-PCR.The products were cloned into the pUCm-T vector,Then we diluted the solution of plasmid and used real-time PCR(SYBR GreenⅠ)to construct the standard curve.

Results:

The correlation coefficient of the standard curve was 0.997 6.The reaction efficiency was 0.862 3.The correlation and the amplify efficiency were good.

Conclusion:

It is successful to construct the standard curve for real-time PCR detecting the tissue expression level of Angptl3 in SD rat.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Chongqing Medical University Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Chongqing Medical University Year: 2003 Type: Article