Modification of collagen digestion method on isolation and culture of rat preadipocyte / 重庆医科大学学报

ABSTRACT

Objective:To establish a better method for isolating and culturing preadipocytes from rat white adipose tissue.Methods:The adipose tissue was digested by collagenⅠand preadipocytes were collected by centrifugations and filtrations.Cells were cultured using basic and differentiation media.Preadipocytes were detected by morphologic method,auxodrome study and stained with Oil red O for determining the cell identity.Results:Using our modified method,primary preadipocytes can be harvested in greater amount compared with the original method.The primary preadipocytes could differentiate into adipocytes under the induction of differentiation medium.The preadipocytes obtained by our method had the characteristics of preadipocytes.Conclusion:We have established an efficient preadipocyte culture method.Our medication method is better than the original method.