The Experimental Study on the relation of Centrosome Hyperamplification and P53 genetic mutation in Bladder Cancer / 重庆医科大学学报

ABSTRACT

Objective:To explore the relation of the abnormal condition of centrosome and p53 genetic mutation of bladder cancer cells,its effect on the onset and progress of the transitional cell carcinoma of bladder because of p53 genetic mutation. Methods: 76 transitional cell carcinoma of bladder were examined.The centrosome was stained by indirect immunofluorescence methods,and the primary antibody was monoclonal antibody against pericentrin.The p53 was stained by SP immunohistochemical methods and the primary antibody was monoclonal antibody against p53.Results: Through statistical analysis,detection of CH or p53 expression was useful in providing prognostic information in bladder cancer. There existed a positive correlated(r=0.707,P