The Induction of Apoptosis by Fas Antibody, IFN - delta, IL - 1 alpha in Normal Human Keratinocytes and KB cells / 대한피부과학회지
Korean Journal of Dermatology
;
: 273-278, 1997.
Article
in Korean
| WPRIM
| ID: wpr-57990
ABSTRACT
BACKGROUND:
The Fas antigen is a cell surface molecule that mediates apoptosis in many cell types. Matsues group indicated that keratinocytes constitutively express the Fas antigen and apoptosis was induced only on pretreatment with interferon-r (IFN-y) in cultured normal human keratinocytes (NHK).OBJECTIVE:
We undertook this study to determine the induction of apoptosis by Fas antibody alone and/or in combination with IFN y, IL-1a in normal human keratinocytes (NHK) and transitional epithelioma cell lines (KB cell) which had lower levels of intracellular IL-1 receptor antago- nists (IL-1ra ).METHODS:
We used cultured NHK and KB cells. Each cell was treated with IFN-r, IL-la and Fas antibody for induction of apoptosis. For quantifying the apoptosis, index fluorescent DNA- binding dyes were used.Result:
Fas antibody alone could induce apoptosis not only in KB cells but also in NHK cells. The combination of Fas antibody and IFN-r enhanced the induction of apoptosis in NHK and KB cells. The IL-la alone could induce apoptosis only in KB cells which had relatively small amounts of IL-1ra compared to NHK.CONCLUSION:
Our result may indicate that Fas antigen in human keratinocytes can regulate normal epidermal cellular differentiation and proliferation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Carcinoma
/
KB Cells
/
Keratinocytes
/
Cell Line
/
Interleukin-1
/
Apoptosis
/
Fas Receptor
/
Coloring Agents
/
Interleukin 1 Receptor Antagonist Protein
/
Interleukin-1alpha
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
1997
Type:
Article
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