Effects of cyclooxygenase-2 inhibitor NS398 on proliferation and apoptosis of renal cell carcinoma cells / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-579903
ABSTRACT
0bjectiveTo study the proliferative and apoptotic effects of NS398,a selective COX-2 inhibitor,on renal carcinoma cell line 786-O in vitro. Methods:
786-O cells were treated with different concentrations of NS398 for24,48,72 and 96 h.The proliferation of 786-O cells was studied by MTT assay.Immuno cytochemistry was used to detect the expressions of COX-2 . The expression of COX-2 mRNA was analyzed by reverse transcription-polymerase chain raction(RT-PCR).Flow cytometric analysis was used to detect the apoptosis status of 786-O cells.Results:
NS398 resulted in significant antiproliferative effects on 786-O cells in dose and time dependent manner. Immunocytochemistry showed the expressions of COX-2 were significantly inhibited by NS398. RT-PCR showed the expressions of COX-2 were significantly inhibited by NS398. Apoptosis rate were 31.5%?2.1% and 14.3%?1.4%,respectively in the 786-O cells treated with NS398 in different concentrations of 180 ?umol/L and 30 ?mol/L,which was significantly higher than in the control group 2.1%?0.4%.Conclusion:
NS398 could effectively suppress the growth of 786-O cells and induce apoptosis,the mechanism of which probably relates to its inhabited expression of COX-2.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2007
Type:
Article
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