Effect of exogenous S100A6 on human osteosarcoma cell line U2OS proliferation,apoptosis and ?-catenin expression / 重庆医科大学学报

ABSTRACT

Objective: we investigated the biological effects of exogenous S100A6 on osteosarcoma cell line U2OS and explored its possible mechanism. Methods:MTT assay was used to determine S100A6 influence on U2OS cells proliferation and their conce ntration-dependent relationship. The trypan blue exclusion assay was used to observe S100A6 inhibitory effect on U2OS cells proliferation in a time-dependent manner;Hoechst staining was used to determine cell apoptosis;Western blot and immunocytochemical methods to determine expressions of cyto-?-catenin. Results:We found that ,on the first three days,OD values in cells subjected to the recombinant protein S100A6(GST-S100A6)with the concentrations of 30,100,300 and 1 000?g/ml respectively were obviously lower than those in the GST group,by 20.5%,23.7%,32.2% and 36.8% accordingly(P