Construction and expression of the eukaryotic expression plasmid of Human pEGFP/H2B / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-580009
ABSTRACT
Objective:
To construct the eukaryotic expression plasmid of EGFP/H2B and express it in the Human embryo kidney cells(HEK293).Methods:
Part H2B gene was obtained by RT-PCR from cultured HEK293 cell's whole RNA.The sequence encoding H2B was cloned into pEGFP-C1 to construct the eukaryotic expression vector.After confirmed by enzymatic digestion and sequencing,the recombination plasmid was transfected to HEK293 cells by lipofectamine technology for observation of the fusion protein's expression.Results:
The recombination plasmid of pEGFP/H2B was constructed successfully.Compared with the dispersing green fluorescence among the whole cells in the control cells transfected with the mock plasmid(pEGFP-C1),the green fluorescence was concentrated in the nuclei of HEK293 cells transfected with pEGFP/H2B and distributed with the chromosomes in the mitotic cells.Conclusions:
The successful construction of pEGFP/H2B recombination plasmids establishes an available survey system to study the chromosome segregation mechanism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS