Modification of arterialized orthotopic liver transplantation in rats / 重庆医科大学学报

ABSTRACT

Objective:To improve the surgical technique to establish the arterialized orthotopic liver transplantion in rat.Methods:On the basis of two-cuff technique of Kamada,we improved the techniques of separaring,perfusing donor liver,the anastomosis of the suprahepatic inferior vena cava,and so on.And used the modified "stent" method to reestablish the hepatic artery.Results:80 cases of operation were completed.The time of donor operation,recipient operation and anhepatic time were(40?3) min,(50?5)min,and(16?2)min,respectively.The time of reestablishing suprahepatic inferior vena cava and hepatic artery were(6~9) min,(2~3) min,respectively.The survival rate at 48 h,1 week and 1 month were 96.3%,93.7%,and 87.5%,respectively.Conclusion:The modified method can enhance the stability and survival rate,which is an ideal method for establishing the arterialized orthotopic liver transplantation model in rat.