HPLC Fingerprint Analysis of Medicinal Slices of Radix et Rhizoma Rhei / 中药新药与临床药理

ABSTRACT

Objective To establish a fingerprint analysis method for the medicinal slices of Radix et Rhizoma Rhei.Methods HPLC with ZORBAX Eclipse XDB-C18 was applied.The chromatographic conditions were as followsacetonitrile-0.1 %H3PO4 solution(gradient alution) as a mobile phase and detection wavelength at 254 nm,flow rate at 1 mL?min-1,and column temperature being 40 ℃.Results Twenty-five common peaks were separated from 10 batches of medicinal slices of Radix et Rhizoma Rhei.Conclusion The method is reliable and accurate,and can be used as a quality control method for the medicinal material of Radix et Rhizoma Rhei.