Effect of baohuoside-Ⅰ on proliferation and cell cycle of human esophageal carcinoma cell Eca-109 / 中草药

ABSTRACT

Objective Effect of baohuoside-Ⅰ from Cortex Periplocae on cell cycle of human esophageal carcinoma cell Eca-109 and its mechanism were studied.Methods After treatment with baohuoside-Ⅰ at different concentration(12.5,25,and 50 ?g/mL)for 24,48,and 72 h,the inhibitory effect on proliferation of Eca-109 cells was analyzed by MTT method.After treatment with baohuoside-Ⅰ under different concentration(12.5,25,and 50 ?g/mL)for 48 h,cell cycle of Eca-109 cells were measured with flow cytometry(FCM);The expression of Cyclin B1 mRNA was detected by RT-PCR technique.The expression of Cyclin B1 protein was detected by Western blotting.Results Baohuoside-Ⅰ of 25 and 50 ?g/mL inhibited the proliferation of Eca-109 cells significantly in an effect-concentration manner(P