Determination of Chrysophanol, Emodin and Rhein in Huangzhi Capsules by TLC-scanning / 中国中医药信息杂志

ABSTRACT

Objective To establish a method for determination of chrysophanol,emodin and rhein in Huangzhi capsules.Method Chrysophanol,emodin and rhein in Huangzhi capsules was determined by TLC-scanning with a mixture of n-hexane-ethylacetate-formic acid(30∶10∶0.5) as the developing system.The detection wavelength was 435 nm and 610 nm.Results The calibraction curve of chrysophanol,emodin and rhein was linear in the range of 0.014 65～0.073 25,0.013 35～0.066 75,0.012～0.072 ?g respectively.Conclusion The method was simple,accurate with good repeatability,and suitable for the content determination of chrysophanol,emodin and rhein in Huangzhi capsules.