Determination of amino acids in plasma of AIDS patients and healthy controls by HPLC with pre-column derivatization / 中国艾滋病性病

ABSTRACT

Objective To study the metabolism of amino acids in AIDS patients for the purpose of providing appropriate nutritional support.Method High performance liquid chromatography combined with pre-column derivatization technology was used to detect free amino acids in plasma from 22 untreated AIDS patients,16 HAART treated AIDS patients and 30 healthy controls.Statistic analysis was performed through SPSS software.Results The concentration of alanine(Ala) was significantly increased and the concentration of phenoalanine(Phe) was remarkably decreased in AIDS patients as compared with the healthy controls(P