shRNA-mediated interference of gene expression PLC? downturn on T24 bladder cancer cells / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-580240
ABSTRACT
Objective:
To investigate whether PLC? gene down regulation by RNA interference(RNAi) leads to inhibition of proliferation in human bladder carcinoma T24 cell.Methods:
The shRNA recombinant plasmids targeting to PLC? gene was constructed and transfected into bladder carcinoma T24 cell with Lipofectamine 2000.RT-PCR was used to monitor the validity of specific s h R N A in down regulation of PLC?.Then MTT assay was performed for detecting cell proliferation,the changes of PCNA were analyzed by immunocytochemical method,Electron microscope was used to observe the morphological changes.Results:
The specific PLC? shRNA was confirmed to be efficient in silencing PLC? expression.PLC? gene down regulation by the shRNA recombinant plasmids inhibition cell proliferation rate about 78.01%,They were significantly different from that of control group(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2007
Type:
Article
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