The primary study on expression and function of PLC? in INS-1 cell lines / 重庆医科大学学报

ABSTRACT

Objective:To investigate the expression of PLC?in INS-1,and the role of PLC?in glucose-stimulated insulin secretion(GSIS).Methods:(1)The six primers of PLC isoforms were designed.The template of INS-1's RNA was collected,RT-PCR was used to analyse the expression of PLC isoforms.(2)The glucose concentration were set at 10,20,40,80,100 mmol/L,and stimulated INS-1 respectively.The content of insulin in INS-1's supernatant liquid was detected.The time groups were set as 20,40,60,80,120 min.The content of insulin in INS-1's supernatant liquid was detected.(3)After the stimulus of glucose,RT-PCR and Western Blot were used to detect the expression of PLC?.Results:(1) PLC?,PLC?,PLC?,PLC?,PLC?were expressed in INS-1.(2) Exposure of INS-1 to 40mM glucose for 60 min,the content of insulin secretion reached the maximum and RT-PCR and Western Blot detected that the expression of PLC?was raised.Conclusion:(1)PLC?was expressed in INS-1.(2)Under the stimulation of glucose,the PLC?'s expression was augmented,so PLC? was probably participated in the signal transduction pathway of GSIS in INS-1.