Fingerprinting Analysis of Radix Salviae Miltiorrhizae with Ultra-high Performance Liquid Chromatography / 广州中医药大学学报

ABSTRACT

Objective To establish an ultra-high performance liquid chromatographic fingerprinting analysis method for the hydrophilic component of salvianolic acids and the lipophilic component of tanshinones in Radix Salviae Miltiorrhizae(RSM),and to supply a rapid and scientific method for quality control of RSM.Methods The chromatographic conditions were as follows Acquity C18 BEH(2.1mm?100 mm,1.7 ?m)chromatographic column at 30℃,gradient elution with acetonitrile(A) and 0.4% formic acid(B)(90%~50% A for 0~10 min,75% A for 15 min),the flow rate being 0.5 mL?min-1 and UV detection wavelength set at 280nm.Results The chromatograms of the hydrophilic component and the lipophilic component were obtained within 15min,peak volume being 85.There were 20 peaks separated completely and identified by using HPLC-diode array detection-electrospray ionization-mass spectrometry.Conclusion The developed method is proved practicable,rapid and reliable for quality control of RSM and its preparations.