Influence of matrine on cyclin in U937 cell strain / 中成药

ABSTRACT

AIM: To investigate the influence of matrine on cyclin E and cyclin A in U937 cell strain. METHODS: The inhibitory effect of matrine on proliferation in U937 cell strain was observed by MTT.The distribution of cell cycle was measured by flow cytometry.Immunocytochemical method and Western blot were used to detect the protein expression of cyclin E and cyclin A. RESULTS: After being treated with matrine,the proliferation of U937 cell strain was inhibited significantly,the inhibiting effect conformed to time and dose-dependence,cells were arrested in S-phase.The expression of cyclin E and cyclin A was down-regulated significantly in a dose-dependence manner. CONCLUSION: Matrine could reduce the expression of cyclin E and cyclin A and inhibit the proliferation in U937 cell strain.