Cloning of 3-dehydroquinate synthase cDNA from Echinacea angustifolia and its tissue expression characteristics / 中草药

ABSTRACT

Objective To clone 3-dehydroquinate synthase cDNA from Echinacea angustifolia and investigate its tissue expression characteristics in various tissues.Methods By using homology cloning and RACE-PCR,the cDNA encoding 3-dehydroquinate synthase was amplified with cDNA library of cultured plantlets as the template.The specificity expression profile in different tissues including roots,stems, leaves,and flowers of E.angustifolia was investigated by semi-quantitative RT-PCR as well.Results The full length cDNA of 3-dehydroquinate synthase(named as EanaroB) had 1 424 bp with an open reading frame encoding 442 amino acids of protein and its EanaroB was around 80%homologous with the sequences of 3-dehydroquinate synthase from other plants.The sequence was reported to the GeneBank and coded as EU293857.The results of semi-quantitative RT-PCR indicated that the expression of EanaroB was detected in different tissues,while only the expression in leaves and flowers reached to a high level. Conclusion The cDNA encoding EU293857 from E.angustifolia is cloned and reported.This work underlays the first step for exploring the pathway of caffeic acid derivatives biosynthesis and improving the content of caffeic acid derivatives including phenylethanoid glycosides.