Simultaneous determination of berberine hydrochloride,palmatine hydrochloride and rhaponticin in Sanhuang Tablets by RP-HPLC / 中成药

ABSTRACT

AIM:To determine the contents of berberine hydrochloride,palmatine hydrochloride and rhaponticin in Sanhuang Tablets(Radix et Rhizoma Rhei,berberine hydrochloride,extract of Radix Scutellariae) by RP-HPLC. METHODS:RP-HPLC method with Shimadzu TM column(250 mm?4.6 mm,5 ?m) was used. The gradient elution mixture mobile phase consisted of acetonitrile and 0.05% Lauryl sodium sulfate(0.1% phosphoric acid). The flow rate was 1.0 mL/min. The detection wavelength was set at 334 nm. The temperature of column was at 25 ℃. RESULTS:The calibration curves of berberine hydrochloride,palmatine hydrochloride and rhaponticin were linear within the ranges of 0.05-1.04 ?g(r=0.999 9),0.02-0.49 ?g(r=0.999 8),0.05-1.02 ?g(r=0.999 9),respectively. The average recoveries (n=6) were 98.7%,97.2%,99.3%,respectively. CONCLUSION:The method of the operation is accurate,reliable and can be used to control the quality of Sanhuang Tablets.