Triglyceride level changes and potential mechanism on alcoholic fatty liver cell model induced in vitro:with or without diammonium glycyrrhizinate / 重庆医科大学学报

ABSTRACT

Objective:To study the triglyceride level changes with or without Diammounium Glycyrrhizinate(DIG)and the potential mechanism on alcoholic fatty liver cell model induced in vitro.Methods:MTT assay was used to screen the optimal concentration of ethanol and DIG injection.Ultra-microstructure was observed under transmission electron microscope.Intracellular triglyceride concentration was measured by hol-automatic biochemistry.Expressions of peroxisome proliferator-activated receptor-gamma (PPAR?),sterol regulatory element binding protein-1(SREBP-1)and SREBP cleavage activating protein(SCAP)were determined by immunocyto-chemistry(ICC).Results:1.The screened optimal DIG concentration was 2.273?g/ml.2.Masses of lipid droplets in cytoplasm were observed under transmission electron microscope.3.Hol-automatic biochemistry measured intracellular triglyceride concentration to be(1.84?0.16)mmol/L and(1.05?0.12)mmol/L respectively in Ld30 group and DIG group,and the difference was statistically significant(P