Cloning and sequence analysis of a novel phenylalanine ammonia-lyase gene from Astragalus membranaceus / 中草药

ABSTRACT

Objective To clone and sequence the cDNA encoding phenylalanine ammonia-lyase(PAL)gene from Astragalus membranaceus.Methods RT-PCR and RACE Techniques were used to clone a phenylalanine ammonia-lyase gene from A.membranaceus roots with the total RNA as the template.Results The cloned gene named as AmPAL and the Genbank registry number is EF567076.Squence analysis showed that the full-length of AmPAL cDNA was 2 650 bp,including a 2 154 bp open reading frame(ORF).AmPAL was a new number of PAL family that consisted of 718 amino acids with prediated mole-cular weight of 7.805?104 and isoelectric point(PI)of 5.96.At the same time,AmPAL had the homo-logy with PAL of known leguminous plants and shared above 80% identity of amino acid sequences.Conclusion It is the first report that a novel PAL gene is cloned from A.membranaceus.This work lays a foundation for regulating phenylpropanoid pathway of medical plant with AmPAL.