Determination of [6]-Gingerol in Rat Plasma by HPLC-UV / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)1993.
Article
in Chinese
| WPRIM
| ID: wpr-580718
ABSTRACT
Objective To develop a HPLC-UV method for the determination of [6]-gingerol in the rat plasma.Methods [6]-Gingerol was extracted from the rat plasma by using liquid-liquid extraction,then was separated on a Hypersil C18 column(250 mm?4.6 mm,5 ?m).The mobile phase was acetonitrile and water(4555,v/v) with a flow-rate of 1.0 mL/min.The eluate from the HPLC column was monitored by the spectrophotometric detector at 280 nm.The injection volume was 20 ?L.[6]-Gingerol was identified based on its retention time compared with the reference standard.Results The linear calibration curve was obtained in the concentration range of 0.25~5.0 ?g/mL.The low quantitative limit was 0.1 ?g /mL.The RSD of inter-day was
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Type:
Article
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