Effects and mechanism of triptolide on retinal gonlion cells of rats with EAE / 重庆医科大学学报

ABSTRACT

Objective:To observe the effects of triptolide(TP) on retinal gonlion cells of SD rats with experimental autoimmune encephalomyelitis(EAE),and to analyze its possible mechanism.Methods:EAE models were induced in SD rats,the TP group received triptolidedayly intraperitoneal injection from immunization day to 14 days.The pathological changes in optic nerve were observed,the expression of MCP-1,GFAP and the apoptosis of RGCs in retina were detected.Results:Demyelination and inflammatory cell infiltration showed in optic nerve,MCP-1 appearred in the retina at the initial stage,and begine to decline after onset 7 days.The expression of MCP-1 and GFAP were lower in TP group,compared with the EAE group with statistically significant difference(P0.05).Conclusion:Triptolide inhibit the expression of MCP-1 and GFAP,have no significant effects on the apoptosis of RGCs.