The relation between poly(ADP-ribose) glycohydrolase,poly(ADP-ribose) polymerase and AKT phosphorylation in colorectal carcinoma cell / 重庆医科大学学报

ABSTRACT

Objective:Poly(ADP-ribose) glycohydrolase(PARG) plays an important role in the inflammation via regulating Poly(ADP-ribose) polymerase(PARP) and AKT phosphorylation,but it is not clear that how it in tumour.The current study was desiged to study the relationship between PARG,PARP,NF-?B and AKT phosphorylation in colorectal carcinoma cell lines LOVO.Methods:Western blot was used to test the expression of PARG,PARP,NF-?B,AKT and PI-AKT473.Gallotannin(GLTN) was served as PARG inhibitor.Results:The expression of PARG,PARP and NF-?B in gallotannin-treated LOVO was weaker than that in the control groups(gallotannin-untreated groups)(P=0.0068,P