Experimental study of tacrolimus for experimental autoimmune encephalomyelitis in rats / 重庆医科大学学报

ABSTRACT

Objective:To explore the efficacy and therapeutic mechanism of immune inhibitor tacrolimus(FK506) for experimental autoimmune encephalomyelitis rats and compare the efficacy of FK506 and confirmed traditional methyllprednisolone.Methods:the guinea pig spinal cord homogenate in complete freund's adjuvant was used to induce the acute EAE in SD rats FK506 at the dosage of 1 mg/kg and 0.5 mg/kg were intraperitoneally injected to EAE rats respetively for daily for ten days.Clinical features were observed,their peripheral blood lymphocytes CD3,CD4,CD8 and NK subsets were counted,pathology changes of brain and spinal cord were observed,and the evidence of nerve regeneration was shown by immunohistochemistry stain.Result:FK506 could relieve remits the clinical phenonoue of EAE promote the climical and release and recoveny of EAE,decrease the inflammatory infiltration in the CNS,decrease demyelination and axonal damage in CNS,promote for axons regeneration.Furthermore,no replase after stopping drug was observed.Conclusion:FK506 as a immune inhibitor can be used the Autoimmune disease,such as EAE.