Determination of Capsaicin and Dihydrocapsaicin in General Alkaloids of Capsaicin by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish an HPLC method to determine the two principal constituent-capsaicin and dihydrocapsaicin in general alkaloids of Capsaicin.Method The analysis was performed on C18 column(4.6 mm?250 mm,5 ?m),with methanol-water(7030) as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was set at 280 nm.Result The linear relationships of capsaicin and dihydrocapsaicin were within the range of 1.955～7.820 ?g(r=0.999 9) and 2.709～7.224 ?g(r= 0.995 5),respectively.The average recoveries were 101.57% and 108.87%,respectively.Conclusion The method is convenient,the result is accurate and reliable,and can be used to determine the content of two principal constituent in general alkaloids of Capsaicin.