Correlation between expression of HPV16/18E6、cyclinD1、hTERT and development of laryngeal carcinoma / 重庆医科大学学报

ABSTRACT

Objective:To evaluate the role of human papillomavirus(HPV)16/18E6,cyclin D1,and human telomerase transcriptase(hTERT)in the development of laryngeal carcinoma by detecting the expression of these three genes.Methods:Immunohistochemistry was used to detect the levels of HPV16/18E6,cyclin D1,and hTERT in 42 cases of paraffin-embedded tissues.Results:(1)The positive expression ratios of HPV16/18E6,cyclinD1,and hTERT in the laryngeal carcinoma group were 42.9%(18/42),50%(21/42),and 83.3%(35/42),respectively.These values were significantly higher than that in the control group.(2)The increase in the expression of HPV16/18E6 and cyclinD1 was associated with T stage advance and lymph node metastases.The expression of hTERT increased along with T stage advance,but not with lymph nodes metastases.The changes in the expression of HPV16/18E6,cyclinD1,and hTERT were not associated with gender and age.(3)Positive correlation was observed between HPV16/18E6 and cyclinD1 as well as between HPV16/18E6 and hTERT in laryngeal carcinoma.Conclusion:(1)The expression of HPV16/18E6,cyclin D1,and hTERT may be associated with the development of laryngeal carcinoma.(2)There may be interactions between HPV16/18E6 and cyclinD1,between HPV16/18E6 and hTERT.These interactions may induce the development of laryngeal carcinoma.The information provided by this study may be important for further investigation into the relationship between HPV16/18 and laryngeal carcinoma.