Identification of clematis raw drugs by HPLC fingerprint / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-580962
ABSTRACT
AIM:
To establish HPLC fingerprint for the root and rhizome of Clematis uncinata Champ and to compare the differences of clematis and Clematis uncinata in fingerprint.METHODS:
Based on 10 batches of Clematis unciuata Champ,its chromatographic seperation was performed on Diamonsil C18 (250 mm ? 4. 6 mm,5 ?m)with a mobile phase consisting of acetonitrile -0.05% phosphoric acid,gradient eluent,at the flow rate of 0. 8 mL/min. The UV detection was set at 210 nm.RESULTS:
The mutual mode to HPLC-UV fingerprints was set up,and the 23 mutual peaks were indicated. The similarities were compared among Cleuatis uncinata Champ and substitutes collected from different sources there were apparent difference in fingerprint.CONCLUSION:
The method is stable and reliable with a good reproducibility and provides a reference standard for identifying medicinal clematis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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