Inhibition of Cell Growth of Human Ovarian Cancer by Xiaoaiping Injection via Akt Signal Pathway / 中国中医药信息杂志

ABSTRACT

Objective To study the effect of Xiaoaiping injection on Caov-3 human ovarian cancer cells and its mechanisms. Methods After treatment with Xiaoaiping injection, viability of Caov-3 cells determined by MTT method. Phase contrast microscopy was used to observed the morphological changes of Caov-3 cells. Cell cycle was assessed by FACS. Cell signaling pathway protein-Akt and pAkt, and cell cycle associated protein-p27 were measured by western blot. Results Xiaoaiping injection inhibited the growth of Caov-3 human ovarian cancer cells in a dose and time dependent manner. Xiaoaiping injection induced G0/G1 phase arrest of Caov-3 cells, accompanied by pAkt down-regulation and p27 up-regulation. Conclusion Xiaoaiping injection can inhibit the proliferation of Caov-3 human ovarian cancer cells by inhibiting PI3K/Akt signaling pathway.