Simultaneous Determination of Five Contents of Rhubarb in Huangxiong Kangshuan Capsule by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish an HPLC method for the determination of Aloeemodin,Rhein,Chrysophanol,Emodin and Physcione in Huangxiong Kangshuan Capsule.Methods The determination was performed on Kromasil-C18 column(250 mm?4.6 mm,5 ?m) with mobile phase consisted of methanol-0.1% phosphoric acid(8515) at a flow rate of 1.0 mL/min.Column temperature was 25 ℃.The UV detection wavelength was set at 254 nm.Results The calibration curves were linear in the range of 0.230～3.68 ?g(r=0.999 8) for Aloeemodin,0.224～3.584 ?g(r=0.999 9) for Rhein,0.223～3.568 ?g(r= 0.999 8) for Emodin,0.257～4.112 ?g(r=0.999 9) for Chrysophanol and 0.287～4.592 ?g(r=0.999 8) for Physcion.The average recovery was 98.48%,98.10%,99.08%,100.34%,97.52% and RSD was 1.39%,1.54%,1.44%,2.40%,2.03% respectively.Conclusion The determination method is simple,reliable,accurate,and it can be used for quality control of Huangxiong Kangshuan Capsule.