HPLC determination of glycoside in Xueshuantong Injection / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-581081
ABSTRACT
AIM:
To establish an HPLC for determining notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 in Xueshuantong Injection (total saponin of Radix et Rhizoma Notoginseng).METHODS:
The analysis was performed on UltimateTM XB C18 column(4.6 mm ? 250 mm,5 ?m) with a gradient mobile phase of acetonitrile(A)water(B) (0-30 min,20% A→38% A;30-31 min 38% A→20% A;31-40 min,20% A) at a flow rate of 1.0 mL/min.The column temperature was maintained at 40 ℃,and the detection wavelength was set at 203 nm.RESULTS:
The linear range of notoginsenoside R1 fell within 0.40 -4.01 ?g (r =0.999 9 ) and the average recovery was 99.36 % (RSD = 0.72% ,n = 6).The linear range of ginsenoside Rg1 fell within 1.47-14.69 ?g (r = 0.999 9) and the average recovery rate was 99.41% (RSD = 0.52% ,n = 6).The linear range of ginsenoside Rb1 fell within 1.50-15.03 ?g(r = 0.999 9) and the average recovery rate was 99.47% (RSD = 0.50% ,n = 6).CONCLUSION:
This method is accurate,simple,rapid and can be used for the quality control of Xueshuantong Injection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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