RP-HPLC determination of paeoniflorin,baicalin,palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-581082
ABSTRACT
AIM:
To develop an HPLC method for analyzing the contents of paeoniflorin,baicalin,palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules(Praeparata Radix Rehmannial,Rhizoma Coptidis,Radix Paeoniae,Radix Scutellariae,Colla Asini corii,Poria).METHODS:
Four compounds were determirned with an Agilent ZORBAX SB C18 column(4.6 mm ? 250 mm,5 ?m).Acetonitrile-0.4% phosphoric acid was used as the mobile phase at a flow rate of 1.0 mL/min.The mobile phase was in linear gradient elution.The detection wavelength was 230 nm.RESULTS:
The linear ranges were within 0.016 58-0.331 6 ?g(r = 0.999 9) for paeoniflorin,0.059 80-1.196 0 ?g(r = 0.999 9)for baicalin,0.012 91-0.258 2 ?g(r = 0.999 9)for palmatine hydrochloride,0.041 98-0.839 6 ?g(r = 0.999 9) for berberine hydrochloride.The average recoveries (n = 6) were 98.91% (RSD = 1.29% ),99.20% (RSD = 0.92% ),97.86% (RSD = 0.89% ),98.63% (RSD = 0.62% ),respectively.CONCLUSION:
The method is simple,reliable,sensitive and can be applied to having controls over the preparation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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