Inhibition of gastric cancer cells invasion and metastasis by Darbufelone in vitro / 重庆医科大学学报

ABSTRACT

Objective:To study the effects of Darbufelone on the invasion and metastasis of human gastric cancer cells SGC-7901 in vitro. Methods:The adhesion ability was determined by MTT.Invasion and metastasis was observed in Transwell Chamber membrane invasion culture system.The capability of SGC-7901 cell condition medium in inducing endothelial cell to form blood vessel in 24 well plate was detected.RT-PCR and cel1-immunohistochemistry were used to observe the expressions of OPN and MMP-2.Results:In the membrane invasion culture system,the numbers of invading and migrating SGC-7901 cells were significantly lower in Darbufelone treated groups compared with those in the control group(sP