Separation,purification and preliminary structure analysis of Ramulus Mori polysaccharide / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-581280
ABSTRACT
AIM:
To study the isolation,purification and characterization of Ramulus Mori polysaccharide.METHODS:
Ramulus Mori was extracted by boiling water. The raw extract was precipitated fractionally by alcohol deproteinized,passing through DEAE ion exchange cellulose ( DEAE-52) and SephadexG-100,obtained RMPS1 and RMPS2. The composition and characterization of Ramulus Mori polysaccharide were researched by TLC、IR、 GC、HPLC and smith degradation.RESULTS:
The molecular weight of RMPS1 and RMPS2 were 5. 8 ? 105 and 6. 5 ? 105; RMPS1was made up of rhammose、arabinose、glucose and galactose with the molarity rate of 1. 08 ∶ 1 ∶ 1. 40 ∶ 1. 57; RMPS2 of rhammose、glucose and galactose with the molarity rate of 11. 38 ∶ 1 ∶ 1. 35. Smith degradation showed that the main linkage form in RMPS1 and RMPS2 was 1→2 and 1→4 glycosidic linkages,But some 1→3 glycosidic linkages also existed in the molecules; infrared spectrum showed that both had the polysaccharide characteristic absorption peaks.CONCLUSION:
The structures of RMPS1 and RMPS2 are first determined from Ramulus Mori.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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