Determination of narigin,hesperidin and neohesperidin in Weili Tablet by HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-581283
ABSTRACT
AIM:
To develop an HPLC method for determining narigin,hesperidin and neohesperidin in Weili Tablet(Rhizoma Pinelliae,Radix et Rhizoma Gentianae,Fructus Aurantii immaturus etc. ).METHODS:
The analysis was carried out with a Diamonsil C18(4. 6 mm ? 250 mm,5 ?m)column and the mobile phase of acetoni-trile-1% glacical acetic acid solution(1981,v/v). The flow rate was 1. 0 mL/min and column temperature was 30 ℃. The detection wavelength was set at 283 nm.RESULTS:
The calibration curve was linear within the range of 6. 0-60. 0 ?g/mL,2. 24-22. 4 ?g/mL and 10. 2-102. 0 ?g/mL for narigin,hesperidin and neohesperidin,respec-tively. The average recoveries were 100. 2% ,98. 8% and 97. 1% for narigin,hesperidine and neohesperidine,re-spectively.CONCLUSION:
The method is reproducible,sensitive and accurate for determination of narigin,hes-peridin and neohesperidin in Weili Tablet.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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