Photocytotoxic and apoptosis of hypericin-mediated on nasopharyngeal carcinoma cell line of human in vitro / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-581286
ABSTRACT
AIM:
To study effects of hypericin associated with light emitting diode irradiation on nasopharyngeal carcinoma cell line of human in vitro.METHODS:
CNE-2 cells were exposed respectively to different concentration of hypericin,and compared with hematoporphyrin as positive control group,and incubated for 6 h in the dark, then accumulative radiated energe of yellow and red light irradiation equivalent to 5. 67 J/cm2 were given throughout 90 min,killing effect and apoptosis of CNE-2 cells were detected by MTT assay after incubation of 24 h and flow cytometry.RESULTS:
MTT assay showed that cytotoxicity of hypericin and hematoporphyrin with light irradiation presented in dose-dependent way,their IC50 values were 0. 049 and 0. 650 ?g/mL,respectively. Flow cytometry showed that hypericin with light irradiation could block cell growth at S phase and G2 phase,when CNE-2 cells were exposed to hypericin at 0. 20 ?g/mL for 18,28 and 48 h,the apoptosis rate reached at 9. 97% ,72. 19% and 92. 24% .CONCLUSION:
Hypericin with light irradiation could obviously inhibit the growth of CNE-2 cells and induce apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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