Cloning of cDNAs Encoding Extra-cellular Domain of Human IL-6, IL-10, IL-13 and SCF / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1994.
Article
in Chinese
| WPRIM
| ID: wpr-581608
ABSTRACT
Human IL-6,IL-10,IL-13 and SCF cDNAs encoding extra - cellular domain were isolated by reverse transcription polymerase reaction (RT-PCR). These cDNA fragments were inserted into expression vector PBV220 which contains PRP_(L), promoters and SD sequence. Then the E. coli DH5? were tronsformed with the recombinant plasmids. After screening, the clones carrying those recombinant plasmids were identified. SDS-PAEG and biological assay indicated that human IL-6, IL-10 and IL-13 were expressed in E.coli. The expression level of rhIL-6 was as high as 30% of the total bacterial protein.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1994
Type:
Article
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