Reserpine treatment activates AMP activated protein kinase (AMPK)
Natural Product Sciences
;
: 157-161, 2017.
Article
in English
| WPRIM
| ID: wpr-58162
ABSTRACT
Reserpine is a well-known medicine for the treatment of hypertension, however the role of reserpine in cell signaling is not fully understood. Here, we report that reserpine treatment induces the phosphorylation of AMP activated protein kinase (AMPK) at threonine 172 (T172) in PC12 cells. Phosphorylation of AMPK T172 is regulated by upstream signaling molecules, and the increase of phospho-T172 indicates that AMPK is activated. When we examined the FOXO3a dependent transcription by using the FHRE-Luc reporter assay, reserpine treatment repressed the FHRE-Luc reporter activity in a dose dependent manner. Finally, we showed that reserpine treatment induced the phosphorylation of AMPK as well as cell death in MCF-7 cells. These results suggest that AMPK is a potential cellular target of reserpine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Reserpine
/
Threonine
/
PC12 Cells
/
Cell Death
/
AMP-Activated Protein Kinases
/
MCF-7 Cells
/
Hypertension
Limits:
Animals
Language:
English
Journal:
Natural Product Sciences
Year:
2017
Type:
Article
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