Preliminary Report on Preparation of Monoclonal Antibodies to Human Plasma Protein C / 中国输血杂志

ABSTRACT

Eight cell strains of mouse hybridoma named PC 01 to PC 08 have been produced by immu-'nizing the mice with human plasma protein C (HPC) which is purified using physico-chemical method. Each of them has long-term stable ability to secret the monoclonal antibodies. Among them,PC 03 is a conformation-specific monoclonal antibody,and its binding to HPC can be pro-motad distinctly by divalent metal ion. However,the monoclonal antibodies of PC02 and PC03 can noticeably advance the enzymatic activity of HPC,whereas the monoclonal antibody of PC04 has capacity to inhibit the enzymatic activity of HPC.