Targeting T Lymphocytes by Bi-specific Monoclonal Antibodies to Eliminate Tumor Cells in Bone Marrow / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
; (6)1995.
Article
in Zh
| WPRIM
| ID: wpr-581629
Responsible library:
WPRO
ABSTRACT
PC84045 human lung carcinoma cells (PC84045 HLCCs) were mixed with the BM cells activated by anti-CD3 McAb and IL-2 and then CD3-ALTO4 bi-specific McAbs was put into the cell suspension. The mixed cells were cultured for 3 days in presence of IL-2 and IL-3. Tumor cell colony formation method in half-solid culture system was used to assess the purging efficiency (PE) of the activated T cells to PC84045 HLCCs. The ratio of T cells to PC84045 HLCCs was 8 to 1, the PE was 4 Logs. The rario of T cells to PC84045 HLCCs was 16 tol, the PE was 5 Logs. When the purged BM cells were injected into the NC nude mice, none of subcutaneous node was found. The number of the CFU-GM, BFU-E of the purged BM cells was more than 85 % compared with that of the fresh BM cells (P
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1995
Type:
Article