The Effects on Proliferative Activity of IL-2 Gene Modified KS62 Cells by Retroviral-Mediated Gene Transfer / 中国肿瘤生物治疗杂志

ABSTRACT

IL-2 gene was introduced into human erythroleukemic cell line K562 by retroviral vector. After transduction for eighteen days, flow microfluorometric analysis for DNA distribution of both K562/neo and K562/IL-2 cells indicated that transduced cells were accumulated in a state of G2M arrest. Thirty days later, the number of G2+M in K562/neo cells decreased, but proportion of G0/G1 in K562/IL-2 cells was still lower and G2+M was higher than that of nontransduced cells. The proliferative activity assay by MTT method demonstrated that the proliferation of K562/IL-2 was slow following four day culture. The index of mitosis was decreased. The results of light microscopical studies showed many giant and multinucleate giant cells with even more than twenty nuclei in each cell. It was possible that the formation of multinucleate giant cells associated with repeated endomitosis in a single cell. Further analysis of morphometry confirmed that the size of IL-2 gene modified K562 cells was increased. The ratio of giant cells was higher than that of nontransduced K562 cells. We suggested that I1-2 gene transduction might have some regulation effects on proliferative activity of K562 cells. IL-2 gene modified K562 cells might be blocked at a stage of G2.