The Antitumor Effects of Mice Peritoneal Macrophages Transferred IL-2 and IFN-Y Gene in vivo / 中国肿瘤生物治疗杂志

ABSTRACT

The eukaryotic expression vector pREP-8-IL-2 or and pREP-8-IFN-? was injected i.p. into mice by calcium phosphate coprecipitation method and IL-2 gene or IFN-? gene was successfully transfected into peritoneal M?s whose expression products could enhance the cytotoxicity of M?s, which secrete some TNF, IL-1 and NO, activate the nonspecific immunity and inhibit the growth of tumor effectively. In particular, IL-2 gene in combination with IFN-? gene transfection were successfully transfected into peritoneal M?s whose high expression products not only significantly enhance the M?s cytotoxicity and made it secrete high level TNF, IL-1 and NO, but also activated the CTLs of spleen and initiated specific immunity and nonspecific immunity . This would produce synergic antitumor effects. The results showed that IL-2 and IFN-? gene transfection produced more antitumor effects than IL-2 or IFN-? gene transfection alone.